| 摘要 |
Disclosed is a method of an apparatus for assaying, in assay apparatus, for an analyte in a sample by specifically binding the analyte to a ligand having a detectable tag to form a complex, the presence of which can be determined quantitatively by measuring the amount of tag. In one embodiment, the sample is contacted in that apparatus with a test body of ligands so as to effect the specific binding. The body has at least two differently tagged different ligands in a predetermined ratio, at least a first of which tagged ligands being that which will specifically bind to the analyte. The second tagged ligand is one which will not specifically bind to the analyte or to other moieties in the sample. The relative non-specific binding characteristics of those ligands with respect to said apparatus are in a known or predetermined relationship. Quantitative measurement of those tags coupled to bound quantities of the ligands is then made substantially simultaneously. From the known proportion of ligands in the test body, the known ratio between the non-specific binding characteristics of the ligands to the apparatus, and the quantitative measurement of the bound tags, one can relatively accurately determine the specific binding of the first ligand to the analyte.
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