发明名称 |
A METHOD OF PRODUCING IgG |
摘要 |
A method for producing IgG from plasma for medical applications, comprising at least: (i') removal of albumin resulting in an IgG fraction, (ii') purifying IgG from an IgG fraction, which is derived from the IgG fraction obtained in step (i'), by adsorbing IgG to a cation exchanger and collecting the adsorbed IgG fraction, and (iii') virus inactivation in an IgG fraction derived from the IgG fraction collected in step (ii'). The method is characterized in; (I) concentrating the IgG fraction obtained in step (i'), (II) adjusting pH to 4+/-0.1 in the IgG fraction released from the cation exchanger in step (ii'), and preferably maintaining the pH below 6.0 during the remaining steps of the method; and (III) carrying out the virus inactivation (step iii') by using chemicals at a temperature of 30 DEG C +/- 2 DEG C for at least 4 hours. Anticomplementary activity is typically below 1 CH50/mg immunoglobulin.
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申请公布号 |
WO0172844(A2) |
申请公布日期 |
2001.10.04 |
申请号 |
WO2001EP03624 |
申请日期 |
2001.03.29 |
申请人 |
AMERSHAM PHARMACIA BIOTECH AB;ANDERSSON, INGER;LINDQUIST, LARS-OLOF |
发明人 |
ANDERSSON, INGER;LINDQUIST, LARS-OLOF |
分类号 |
C07K1/18;C07K1/34;C07K16/06;(IPC1-7):C07K16/06;A61K39/395 |
主分类号 |
C07K1/18 |
代理机构 |
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主权项 |
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