| 摘要 |
<p>The present invention concerns a method of using a muscle specific tyrosine kinase receptor molecule (MuSK-R) or a mutated MuSK-R (mMuSK-R) thereof as a selectable marker in mammalian cells, particularly human cells. Preferred markers are mMuSK-Rs incapable of signal transduction and preferably molecules wherein the intracellular domain has been modified by deletion of the signaling region. The invention also relates to a method of identifying genetically modified mammalian cells including introducing a mMuSK-R into a target cell as a selectable marker. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising identifying the transduced cells by incubation of the cells with an antibody which recognizes and binds specifically to a MuSK-R or mMuSK-R thereof.</p> |
