BIOLOGICAL PARTICLE SORTER

摘要

An automated particle sorter includes a fluid flow path (20), which places single biological particles (11) in an optical cuvette (35). An exciting light irradiation system having a light source (30) emits a source of light (32) through the cuvette (35). The light (32) excites a fluorescent substance present on the particle (11), and the emitted light is detected by a light detection apparatus having at least two detection elements (70) for measuring the fluorescence emitted from the fluorescent substance. A light separation element (60) separates the fluorescence from the exciting light (32). A data processor (80) compares the signal received from the fluorescent light, and from the background autofluorescent signal, and according to preset parameters, controls the position of a collection conduit (46) between two set points. The first being a collection set point (41) for the collection of objects having a first phenotype and the second being a set point (42) for the collection of objects having a second phenotype.