摘要 |
<p>Nucleic acids having a novel base whereby nucleic acids can be reversibly ligated to each other by light-irradiation; nucleic acids wherein these nucleic acids have been immobilized; a method of conveniently producing branched nucleic acids and capped nucleic acids by using the above nucleic acids; a method of immobilizing nucleic acids on a solid support by using light without resort to any enzymes or chemical reagents; and a method of purifying and recovering nucleic acids and a method of identifying, detecting and quantifying nucleic acids with the use of the above method. Namely, a method of reversibly ligating nucleic acids conveniently and efficiently by using the light-reactivity of a pyrimidine base having a substituted vinyl group at the 5-position of pyrimidine; nucleic acids therefor; a method of inactivating DNA by using the same; and the like. Nucleic acids having a pyrimidine base having a substituted vinyl group at the 5-position of pyrimidine immobilized on a solid support; methods of immobilizing, purifying, recovering, identifying, detecting or quantifying nucleic acids having specific base sequences by using the above nucleic acids.</p> |