摘要 |
<p>Promoters having been improved so as not to undergo methylation in the course of constructing transformants. The above promoters are DNAs as defined by the following (1) or (2): (1) DNAs containing the base sequences represented by SEQ ID NOS:1 to 4; and (2) DNAs containing base sequences derived from the base sequences represented by SEQ ID NOS:1 to 4 by deletion, addition or insertion of one to several bases, being free from the consecutive sequences represented by CG, CAG, CTG, CCG and CGG in the deleted, added or inserted base sequence, and having a promoter activity. By using these promoters, the expression efficiency of a structural gene of a plant showing weak expression even by using cauliflower mosaic virus 35S promoter, which has been considered as a high-expression promoter for plants, (for example, chrysanthemum) can be elevated.</p> |