发明名称 |
METHOD FOR LIGATING NUCLEIC ACIDS AND MOLECULAR CLONING |
摘要 |
The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nuclei c acid insert molecules by topoisomerase and DNA ligase. The methods provide f or directional and non-directional covalent joining and cloning of nucleic acid molecules. |
申请公布号 |
CA2401632(A1) |
申请公布日期 |
2001.08.30 |
申请号 |
CA20012401632 |
申请日期 |
2001.02.22 |
申请人 |
STRATAGENE |
发明人 |
CARSTENS, CARSTEN-PETER;BAUER, JOHN;GREENER, ALAN L.;GIBBS, RICHARD;JI, HENRY;SORGE, JOSEPH A. |
分类号 |
C12N15/09;C12N15/10;C12N15/66 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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