Verfahren zur Aufreinigung von Nukleinsäuren

摘要

The invention relates to a method for purifying nucleic acids from a nucleic acid containing starting material by means of a porous membrane. According to the inventive method, the optionally pre-purified starting material is mixed with a binding buffer that contains a filler in a concentration sufficient to adjust the final concentration in the mixture of starting material and binding buffer above the value required for precipitating nucleic acids. The binding buffer containing the starting material is left to permeate the membrane, whereby the nucleic acids are selectively bound to the surface or in the pores of the membrane. The membrane is optionally washed, and the nucleic acids are optionally eluted from the membrane in an additional step.