<p>A method of immunoassay of an analyte in a liquid sample comprises the steps of providing a reactive plate in a vessel; adding to the vessel liquid First reagent which reacts with the surface of the plate to become bound thereto; adding the liquid sample containing the analyte under assay to the vessel, whereby the analyte is immunospecifically bound directly or indirectly with said first reagent on said plate; and directly or indirectly assaying the analyte bound to the plate. The plate may be a disc of paper or plastics material with an activated surface. The method may be used for the diagnosis of allergy wherein the first reagent is an allergen and the analyte is an IgE immunospecific thereto. <IMAGE></p>
