| 摘要 |
A combined medication for increasing the capacitation of mammalian sperm is described, comprising two or more agents each of which is selected from the groups consisting of (1) calcitonin, (2) angiotensin II, and (3) a modulator of adenosine receptor activity, which may be fertilization promoting peptide (FPP) or adenosine or a specific adenosine receptor agonist. A preferred combination is calcitonin, FPP (or FPP/adenosine), and angiotensin II. The agents may be formulated as (1) a cream, jelly or pessary containing from 100 nM to 100 mu M FPP or from 10 mu M to 10 mM adenosine, from 5 ng/ml to 5 mu g/ml salmon calcitonin or from 200 ng/ml to 200 mu g/ml of human calcitonin and from 1 nM to 1 mu M angiotensin II, or (2) as a sperm preparation for use in the treatment of human infertility containing from 12.5 to 500 nM FPP or from 0.5 mu M to 100 mu M adenosine, from 0.5 to 150 ng/ml salmon calcitonin or from 2 ng/ml to 1000 ng/ml of human calcitonin, and containing from 0.1 to 100 nM angiotensin II. For agricultural use a sperm preparation may contain concentrations of added FPP from 12.5 nM to 1 mu M or adenosine from 0.5 to 100 mu M, salmon calcitonin from 0.5 to 400 ng/ml or porcine calcitonin from 2 to 1000 ng/ml, and angiotensin II from 0.1 to 100 nM. The use of angiotensin II alone for the preparation of a medicament for stimulating the capacitation of mammalian sperm is also described.
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