摘要 |
Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating the oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.
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申请人 |
THE SCRIPPS RESEARCH INSTITUTE;MAURO, VINCENT, P.;EDELMAN, GERALD, M.;CHAPPELL, GERALD, M.;JONES, FREDERICK, S.;OWENS, GEOFFREY;MEECH, ROBYN |
发明人 |
MAURO, VINCENT, P.;EDELMAN, GERALD, M.;CHAPPELL, GERALD, M.;JONES, FREDERICK, S.;OWENS, GEOFFREY;MEECH, ROBYN |