发明名称 |
DNA ENCODING ASPARTOKINASE III MUTANTS AND THEIR USE FOR THE PRODUCTION OF L-THREONINE BY FERMENTATION |
摘要 |
A DNA containing a gene that codes for aspartokinase III from a bacterium of the genus Escherichia and has in the coding region thereof a variation that releases the inhibition of aspartokinase III feedback by lysine; a recombinant DNA comprising the above DNA joined to a vector DNA capable of autonomous replication in the cell of a bacterium of the genus Escherichia; a micro-organism of the genus Escherichia, that has been transformed by introducing the recombinant DNA into the cells; and a process for producing L-threonine by culturing the micro-organism in a fermentation medium to accumulate L-threonine in the medium and harvesting the same from the medium. The invention process has provided AK III genes originating in a bacterium of the genus Escherichia and sufficiently released from the feedback inhibition caused by lysine. An improved threonine- producing bacterium has been obtained by introducing the above genes into a conventional threonine-producing bacterium. The invention successfully provides a process for producing L-threonine by an improved fermentation method using the above threonine-producing bacterium. |
申请公布号 |
EP0643135(B1) |
申请公布日期 |
2001.08.01 |
申请号 |
EP19930924805 |
申请日期 |
1993.11.10 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
SANO, KONOSUKE;KOJIMA, HIROYUKI;OGAWA, YURI |
分类号 |
C12N1/21;C12N9/12;C12N15/54;C12N15/63;C12P13/08;C12R1/19 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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