| 摘要 |
<p>The present invention relates to a polynucleotide encoding a fusion protein which is stable in a cell, said fusion protein comprising a first (poly)peptide and a second (poly)peptide which co-precipitates a chaperone. The present invention also relates to a vector comprising the polynucleotide of the invention, a host cell comprising the polynucleotide or the vector of the invention, and a method for the production of the fusion protein of the invention. Also described are methods for the production of said first (poly)peptide, of a fusion protein/chaperone complex, and of an antibody directed against said first (poly)peptade, as well as a method of immunizing a subject with the polynucleotide, the vector, the fusion protein, said first (poly)peptide and/or said fusion protein/chaperone complex of the invention. In addition, the present invention relates to a kit and a diagnostic composition comprising the polynucleotide, the vector, the host cell, the fusion protein, the first (poly)peptide, the fusion protein/chaperone complex and/or the antibody of the invention. The present invention, furthermore, relates to a method for the detection of the presence of an epitope comprised in a (poly)peptide. Additionally described is a pharmaceutical composition comprising the polynucleotide, the vector, the fusion protein, the first (poly)peptide, the antibody, and/or the complex of the present invention and, optionally, a pharmaceutically acceptable carrier and/or diluent, said pharmaceutical composition being preferably a vaccine. Finally, the present invention relates to the use of the polynucleotide or the vector of the invention for the production of an antibody directed against said first (poly)peptide, and the use of a (poly)peptide comprising an epitope detected by the method of the present invention or a complex produced by the method of the invention for the production of an antibody.</p> |
