SOMATIC CELL CLONED ANIMAL AND PRODUCING METHOD THEREOF

摘要

PURPOSE: A somatic cell cloned animal and a producing method thereof are provided, thereby the somatic cell cloning technique can be applied to various other mammal animals including human. CONSTITUTION: The method for producing somatic cell cloned animal comprises the steps of: collecting an immature oocyte from a cow and incubating it; separating cell lines from various tissue of a cow; cutting the transparent membrane of the mature oocyte and removing first polar body and 10 to 30% of cytoplasm; transplanting the cell lines as a donor into the nucleus removed oocyte; adding the DC electric shock into the transplanted oocyte to produce the fusion cell; activating the fusion cell in a medium containing chemical materials immediately after electric shock; and second activating and incubating the fusion cell in a drop medium.