| 摘要 |
A method for the qualitative and quantitative detection of damages to DNA, characterised in that it includes the steps of binding the target DNA to a sensitised substrate, subjecting the DNA to the action of a test composition comprising at least one damage-generating product, subjecting the DNA to the action of a composition containing at least one cell extract active in repairing the DNA and containing markers, and detecting directly or indirectly the markers optionally incorporated into the DNA, if the repair has been effective, wherein a washing step is included between the steps and the binding and damaging steps can be reversed. The invention also provides a method for capturing the DNA directly from the cells with a lysis solution and a method for determining the repair modulation effect of some molecules. The method is suitable for detecting DNA damage and the repair inhibition modulating activity of one or more molecules.
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