DNA CHIP MANUFACTURING METHOD

摘要

PROBLEM TO BE SOLVED: To shorten the manufacturing time of DNA chips and to reduce manufacturing cost. SOLUTION: Template oligonucleotide 2 is fixed to the inner wall surface of a capillary tube 4 (A), and a large number of capillary tubes 4 in which template oligonucleotide 2 is fixed to the inner wall surfaces are prepared. Then their one-end sides are two-dimensionally arranged and bundled. A PCR reaction liquid 6 is filled in the capillary tubes 4 (B) to synthesize complementary oligonucleotide 8 by the PCR reaction with the template oligonucleotide 2 as a template (C). Than the PCR reaction liquid 6 is removed D, and the template oligonucleotide 2 is dissociated from the oligonucleotide 8 (E). One faces of the capillary tubes 4 are brought into contact with the surface 10 of a nylon film to make a part of an eluate 12 adhere to the surface 10 of the nylon film and to move the oligonucleotide 8 to the side of the surface 10 of the nylon film and fix it to the surface 10 of the nylon film (F).