发明名称 |
Antibodies, production method of the antibodies, hybridomas which produce the antibodies, production method the hybirdomas and antigen proteins recognized by the antibodies |
摘要 |
The invention is related to antibodies which specifically react with connective tissue type-human mast cells, a production method of the antibodies, hybridomas which produce the antibodies, a production method of the hybridomas and antigen proteins recognized by the antibodies. After cord blood cells were cultured in the presence of SCF and IL-6, they were further cocultured with primary culture of human skin fibroblasts, and connective tissue type-human mast cells were thus obtained. A rat was immunized using the cells, hybridomas were prepared and selected by an ordinary method, and novel monoclonal antibodies were harvested from the culture supernatant of the selected hybridomas. The monoclonal antibodies specifically reacted with connective tissue type-human mast cells.
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申请公布号 |
US6258564(B1) |
申请公布日期 |
2001.07.10 |
申请号 |
US19990363787 |
申请日期 |
1999.07.29 |
申请人 |
MEDICAL & BIOLOGICAL LABORATORIES CO., LTD. |
发明人 |
KAWAI MAKOTO;OKADA TADASHI;ATSUMI FUKIKO |
分类号 |
C12N15/02;A61K39/395;A61P37/08;C07K14/705;C07K16/28;C12N5/00;C12N5/10;C12N5/20;C12N15/00;C12P21/08;C12R1/91;(IPC1-7):C12N15/02;C12P21/02 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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