| 摘要 |
An composition, method and system for identifying novel antimicrobial agents including the steps of, displaying a beta-lactamase inhibitor protein on a virus, contacting the virus with a beta-lactamase binding protein target, selecting for the virus that has a higher affinity for the target and testing the beta-lactamase inhibitor protein for antimicrobial activity, is disclosed. The invention also includes a nucleic acid encoding a fusion protein comprising a beta-lactamase inhibitor protein and an affinity carrier and the protein expressed therefrom. Mutant beta-lactamase inhibitor proteins may be produced, characterized, isolated and expressed in prokaryotic cells and used as antimicrobial agents.
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