| 摘要 |
<p>The present invention includes the cloning of a novel human Cα isoform, identified as the human Cα-s protein on the basis of its similarity to a recently characterized Cα isoform which was identified by purification and peptide sequencing of Cα from ovine sperm [11]. The human Cα-s protein was present in testis and ejaculated sperm and Cα-s localized to the midpiece of human sperm. The present invention includes in this respect a cDNA sequence encoding a Cα-s protein and comprises the specific nucleotide sequence shown in SEQ ID NO:1, (underlined) wherein the said protein is a new splice variant of Cα catalytic subunit of cAMP dependent protein kinase. The present invention is further directed to a vector comprising the said cDNA sequence. The invention also includes a protein characterised by the Cα-s protein specific amino acid sequence shown in SEQ ID NO:2 (underlined). The invention includes further use of Cα-s protein and cDNA sequence in preparation of pharmaceuticals, medicaments to manipulate the motility in sperm or for use as contraceptive, as well as methods and testsystems for screening molecules interacting with Cα-s. The present invention includes also a kit.</p> |
