REAGENTS, METHODS AND KITS FOR DETECTING <i>BACILLUS THURINGIENSIS</i> PROTEINS

摘要

Epitopes, antibodies, methods and kits for the detection of Bt proteins in a sample are provided. The proteins to be detected are the Bt proteins known to those skilled in the art as the Cry1 Ab and Cry1 Ac proteins. The epitopes are antigenic peptides of these Bt proteins and are immunoreactive with the monoclonal antibodies 113L2 and 113I1. The epitopes are isolated or synthesized and administered to animals to produce anti-Bt monoclonal and polyclonal antibodies having superior sensitivity for Bt proteins. The antibodies are useful in immunoassay methods for the detection of genetically modified organisms that have been engineered to include a Bt gene.