发明名称 |
CHARACTERIZATION OF HYBRIDIZED POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS |
摘要 |
The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.
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申请公布号 |
WO0142782(A1) |
申请公布日期 |
2001.06.14 |
申请号 |
WO2000US33357 |
申请日期 |
2000.12.08 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
DENISON, TIMOTHY, J.;SAUER, ALEXIS;GOLOVCHENKO, JENE;MELLER, AMIT;BRANDIN, ERIC;BRANTON, DANIEL |
分类号 |
C07K14/705;C12N9/12;C12Q1/68;G01N27/04;G01N33/68;(IPC1-7):G01N33/483 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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