IMPROVED FLOW CYTOMETER NOZZLE AND FLOW CYTOMETER SAMPLE HANDLING METHODS

摘要

A method of flow cytometry sample processing, comprising the steps of
establishing a sheath
fluid; injecting a sample into said sheath fluid at an injection point;
subjecting said sample to
a first axial motion surface in a nozzle; transitioning to a second axial
motion surface in said
nozzle; subjecting said sample to said second axial motion surface in said
nozzle wherein said
first and said second axial motion surfaces transition with a maximal
acceleration
differentiation; coordinating said maximal acceleration differentiation so as
to not exceed the
practical capabilities of said sample over its length; affirmatively limiting
said maximal
acceleration differentiation so as to not exceed the practical capabilities of
said sample over
its length; exiting said sample from said nozzle; analyzing said sample.