| 摘要 |
Disclosed are an optical flow particle apparatus and method for conducting a particle light scatter-based immunoassay for simultaneously measuring the presence or amount of one or more analytes in a fluid sample, which involves the steps of: (a) combining with the fluid sample, first binding molecule-coated monodisperse microspheres having a resolvable light scatter signal and second binding molecule-coated colloidal particles, or a prior-prepared immunocomplex comprising the monodisperse microspheres, the analyte and the colloidal particles, to form a mixture and to allow a reaction to occur, the reaction being the formation or the decomplexation of the immunocomplex, respectively, so that the thus-reacted mixture includes relative amounts of non-colloidal particles selected from the group consisting of the immunocomplex and uncomplexed microspheres, the relative amounts of the immunocomplex and the uncomplexed microspheres being dependent upon the presence or amount of analyte in the fluid sample; b) illuminating the non-colloidal particles with an incident light source to produce light scatter signals for each of the non-colloidal particles; and c) determining a statistical distribution of the thus-produced light scatter signals so that the distribution can be correlated with the presence or amount of the analyte in the fluid sample. In the case where more than one analytes are detected, each monodisperse microsphere has a resolvable light scatter signal.
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