A METHOD FOR SELECTIVE REMOVAL OF A SUBSTANCE FROM SAMPLES CONTAINING COMPOUNDS HAVING NUCLEIC ACID STRUCTURE

摘要

A method for purifying a desired substance comprising nucleic acid structure by separating substance (I) from substance (II), one of which is the desired substance, both of which have affinity for the same ligand structure, and substance (I) is smaller than substance (II). The method comprises: (i) providing substances I and II in a liquid; (ii) contacting the liquid with a n adsorbent which selectively adsorbs substance I; (iii) recovering the desire d substance; The adsorbent has (a) an interior part which carries a ligand structure that is capable of binding to substances I and II, and is accessib le to substance I, and (b) an outer surface layer that does not adsorb substanc e II, and is more easily penetrated by substance I than by substance II. The u se of certain anion exchangers funtionalized with a plurality of chargeable ami ne groups for the removal and/or purification of nucleic acid vectors. The matrices used should have (A) an increased elution ionic strength for at lea st one of a selection of standard proteins compared to a reference anion exchanger, or (B) have a hydroxy or amino nitrogen at a distance of 2-3 carbons from the amine groups.