发明名称 |
Nucleic acid detection in cytoplasm |
摘要 |
Detection probes labeled with fluorescent dyes, to which are bound nuclear membrane unpermeable molecules via linkers, having base sequences that can hybridize to a target nucleic acid. The probes are introduced into the cytoplasm of a living cell in which the target nucleic acid is present, and the target nucleic acid is detected by measurement of the change in fluorescence of the fluorescent dyes due to the formation of a hybrid of the target nucleic acid and the probes.
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申请公布号 |
US6228592(B1) |
申请公布日期 |
2001.05.08 |
申请号 |
US19990476256 |
申请日期 |
1999.12.30 |
申请人 |
LABORATORY OF MOLECULAR BIOPHOTONICS |
发明人 |
TSUJI AKIHIKO;HIRANO MASAHIKO;KOSHIMOTO HIROYUKI;ISHIBASHI KANAME |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C07H21/02;C07H21/04;C12N15/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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