发明名称 |
Process for producing L-Threonine by fermentation |
摘要 |
<p>A DNA containing a gene which codes for aspartokinase III originating in Escherichia bacteria and which has in the coding region a mutation which releases the feedback inhibition by lysine on the aspartokinase III, a recombinant DNA wherein the DNA is linked to a vector DNA capable of autonomous replication in Escherichia bacteria, a microorganism belonging to the genus Escherichia which has been transformed by the introduction of the above mentioned recombinant DNA into the cells thereof, and a method for the production of L-threonine which comprises culturing the microorganism in a fermentation medium, producing and accumulating L-threonine in the culture medium, and collecting the L-threonine from said culture medium. Escherichia bacteria-derived AK III genes are obtained which sufficiently release the feedback inhibition due to lysine. Introduction of these genes into threonine-producing bacteria results in new threonine-producing bacteria which are much improved in their threonine production over those of the prior art. Use of these threonine-producing bacteria provides a much more excellent method for the production of L-threonine by fermentation than the conventional methods.</p> |
申请公布号 |
EP1020526(A3) |
申请公布日期 |
2001.05.02 |
申请号 |
EP20000105221 |
申请日期 |
1993.11.10 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
SANO, KONOSUKE;KOJIMA, HIROYUKI;OGAWA, YURI |
分类号 |
C12N1/21;C12N9/12;C12N15/54;C12P13/08 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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