| 摘要 |
<p>The present invention provides a method for amplifying and detecting target nucleic acid. One method of present invention comprises Polymerase Chain Reaction (PCR) amplification process with two different standard oligonucleotide primers along with extra number of oligonucleotide primers bound to a solid support. The extra number of oligonucleotide primers bound to the solid support can be co-amplified along with equal or preferably unequal amount of two standard oligonucleotide primers. Another method of present invention involves multi-number of target amplification by periodical addition of the oligonucleotide primers during the amplification process. These processes are useful for specifying the amplified nucleic acid, which allow the simple and convenient format for diagnostic and DNA sequencing.</p> |
