| 摘要 |
The invention concerns a method for the detection of telomerase activity wherein(a) a sample to be tested is provided,(b) a first primer suitable as a telomerase substrate and nucleoside triphosphates are added and the reaction mixture is incubated under conditions under which a primer extension by the telomerase can take place,(c) an amplification of the extension product produced by the telomerase is carried out,(d) the amplification product produced in step (c) is immobilized on a solid phase and(e) the immobilized amplification product is detected qualitatively or/and quantitatively. Furthermore the invention concerns a suitable reagent kit for carrying out the method.
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