| 摘要 |
An expression vector for preparing a library of an antibody variable region, which can express comprising polypeptides containing the H-chain and/or L-chain variable regions of antibodies in the membrane-bound form on the surfaces of eukaryotic cells, which is containing the nucleotide sequence of AKL (formula 1) and/or the nucleotide sequence AKH (formula 2), and which is replicable in the cells:wherein PL and PH represent a promoter, respectively; SL and SH represent a nucleotide sequence coding for a signal peptide respectively; CL represents a nucleotide sequence coding for the L-chain constant region of an antibody; CH represents a nucleotide sequence coding for the H-chain constant region of an antibody or a nucleotide sequence coding for a polypeptide containing at least CH1 of the H-chain constant region of an antibody; AL and AH represent a polyadenylation signal, respectively; "5'-" and "-3'" refer to the 5' side and the 3' side, respectively, of the nucleotide sequence; and ML and MH represent a nucleotide sequence coding for the transmembrane domain, respectively, while either ML or MH may be a mere chemical bond when the expression vector contains the nucleotide sequence of AKL and AKH. Cloning site of R1L, R2L, R1H and R2H is present within or in the vicinity of SL, CL, SH and CH respectively, in order for the nucleotide sequences coding for the L-chain and H-chain variable regions to be readily inserted in between SL and CL, and SH and CH respectively.
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