| 摘要 |
A method is described for screening for the presence or absence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase activity comprising the following steps : (a) preparing a first aqueous mixture containing a protein having a plant-type 6,7-dimethyl-8-ribityllumazine synthase sequence, 5-amino-6-ribitylamino-2,4(1H,3H)pyrimidine-dione and 3,4-dihydroxy-2-butanone 4-phosphate; (b) reacting said first mixture during a predetermined period of time at a predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (c) preparing a second aqueous mixture by including in said first mixture a predetermined amount of a chemical test sample; (d) reacting said second mixture during the predetermined period of time at the predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (e) determining the presence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase by observation of whether the level detected in step (d) is lower than the level detected in step (b). |
