| 摘要 |
PURPOSE: Provided is a process for the production of water-soluble, naturally folded and secreted polypeptides after expression in prokaryotic cells. CONSTITUTION: The process for the production of a water-soluble, naturally folded eukaryotic polypeptide containing two or several cysteines linked by disulfide bridges, comprises the steps of: culturing prokaryotic cells, a) in which the prokaryotic cells contain an expression vector which encodes the polypeptide which contains a prokaryotic signal sequence at the N-terminus, b) under conditions under which the polypeptide is secreted into the periplasm or the medium; and cleaving the signal sequence and isolating the polypeptide from the periplasm or the medium wherein the culture is carried out in the presence of arginine or a compound of the general formula(I): R2-CO-NRR1 in which R and R1 represent hydrogen or a saturated or unsaturated branched or unbranched C1 - C4 alkyl chain and R2 represents hydrogen, NHR1 or a saturated or unsaturated branched or unbranched C1 - C3 alkyl chain. |
