摘要 |
<p>Annealing promoting compounds (APCs) are used to reduce the hybridization specificity of oligonucleotide duplexes containing one or more nucleotide mismatches as compared to perfectly base-paired oligonucleotide duplexes. These APCs are used in methods for the detection of target oligonucleotides that vary in nucleic acid sequence by one or more nucleic acid as compared to a control sequence.</p> |