DNA CONTAINING VARIANT FRT SEQUENCES

摘要

DNA containing variant FRT sequences each undergoing a recombination reaction not with wild type FRT sequences but with the variant FRT sequence of the same sequence in the presence of recombinase FLP; a method of performing highly efficient gene insertion or gene replacement, etc. Namely, DNA containing variant FRT sequences (SEQ ID NOS:2 to 5); DNA containing at least one base replacement in a domain excluding the spacer domains of the above sequences and containing variant FRT sequences which (A) do not undergo any specific DNA recombination reaction with wild type FRT sequences in the presence of recombinase FLP, but (B) undergo a specific DNA recombination reaction with another variant FRT sequence having the same sequence; a cell transformed by the above DNA; a gene replacement method wherein the above DNA is used in the presence of recombinase FLP; a transgenic animal having the above DNA on its chromosome; drugs containing the above DNA; and a method of specific DNA recombination characterized by using two variant FRT sequences (SEQ ID NO:32) and performing a specific DNA recombination reaction in the presence of recombinase FLP.