| 发明名称 |
Method for producing nucleoside-5'-phosphate ester |
| 摘要 |
A method for producing nucleoside-5'-phosphate esters inexpensively and in high yields by phosphorylating a nucleoside with a phospahte group donor using an acid phosphatase having an increased affinity for the nucleoside and/or an increased temperature stability at a pH of pH 3.0 to 5.5, to produce a nucleoside-5'-phosphate ester. Mutant acid phosphatases having increased affinity for nucleosides and/or an enhanced temperature stability are also provided.
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| 申请公布号 |
US6207435(B1) |
申请公布日期 |
2001.03.27 |
| 申请号 |
US19990417090 |
申请日期 |
1999.10.13 |
| 申请人 |
AJINOMOTO CO., INC. |
发明人 |
MIHARA YASUHIRO;UTAGAWA TAKASHI;YAMADA HIDEAKI;ASANO YASUHISA |
| 分类号 |
C12N15/09;C12N1/21;C12N9/16;C12N15/00;C12N15/55;C12P19/30;C12P19/36;C12R1/01;C12R1/185;C12R1/19;C12R1/22;C12R1/425;(IPC1-7):C12N9/16;C12N9/12 |
主分类号 |
C12N15/09 |
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