Partially crosslinking proteins with transglutaminase

摘要

The invention pertains to a process for partially crosslinking proteins using transglutaminase. The transglutaminase is coupled to a material which allows control of the crosslinking process as well as separation of the enzyme from the crosslinked product. The transglutaminase is preferably of bacterial origin, and can be immobilised on a solid support. Crosslinking is controlled in such a manner that from 2 to 50 protein molecules are coupled, corresponding with a molecular weight of the crosslinked product between 10 and 1000 kDa.