CDNA ENCODING C6 BETA-CHEMOKINE LEUKOTACTIN-1(LKN-1) ISOLATED FROM HUMAN

摘要

PURPOSE: The present invention relates to a cDNA coding for a novel protein which belongs to C6 beta -chemokines and attracts subsets of peripheral blood leukocytes, and a process for preparing the said protein by employing expression vector therefor. CONSTITUTION: Open reading frame of the Lkn-1 cDNA encodes 113 amino acids containing a signal peptide of 21 amino acids, and molecular weight of mature protein consisting of 92 amino acids among them is supposed to be 10,162 dalton. A recombinant Lkn-1 which was expressed in E.coli or insect cell employing the said Lkn-1 cDNA and purified, inhibited colony formation significantly, attracted neutrophils, monocytes and lymphocytes to cause chemotaxis, and bound to CCR1 and CCR3 receptors. Accordingly, it was determined that the recombinant Lkn-1 protein can be used as a potential drug for antibody production, the treatment during HIV-1 infection, the protection of bone marrow stem cells during chemotherapy or radiotherapy and the inhibition of leukemia, etc.