METHOD FOR PRODUCING ACTIVE SERINE PROTEASES AND INACTIVE VARIANTS

摘要

The invention relates to a method for producing biologically active serine proteases, isolated serine protease domains and their amino-acid variants in a prokaryotic host. The method is characterised by an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl amino peptidase, by the expression of the serine protease(s) and/or their fragments containing the N-terminal dipeptide helper sequence, optionally as inclusion bodies, and by the renaturation of the expressed proteins and the activation of the serine protease(s) and/or serine protease domains by splitting-off the helper sequence using an exopeptidase.