| 摘要 |
<p>The invention provides a method for sperm preparation and artificial insemination comprising placing a sperm sample in a syringe-like container (4) having a narrow first opening (6) with a removable seal (8), and a wider second opening (10), introducing a plunger-like device (12) into the second opening, wherein the device (12) is provided with a filter element (14) along a bottom surface thereof, the filter leading to a chamber (16) in the plunger (12), and wherein the device is sized for friction-fit engagement with inner walls (18) of the container (4), whereby a portion of smaller components of the sample, including seminal fluid, proteins, prostaglandins and bacteria are forced through the filter (14), into the chamber (16), leaving motile sperm cells and a portion of seminal fluid in the container, at least partially removing the plunger-like device (12) and discarding filtrate collected therein, introducing pure medium into the container to wash sperm cells and dilute seminal fluid remaining therein, depressing the plunger-like device into the container (4) to force at least a portion of the medium and remaining inpurities into chamber thereof, thereby leaving washed motile sperm cells and a portion of the medium in the container, at least partially removing the plunger-like device and discarding filtrate collected therein and utilizing washed motile sperm cells remaining in the container for artificial insemination.</p> |
