摘要 |
The present invention relates to a novel unconventional method for cloning large and multiple segments of DNA into a vector. More specifically the present invention provides nucleic acid sequences for selectively regulating site-specific recombination in favor of insertion of multiple segments of DNA in a plant transformation vector. In particular, the invention relates to the use of <i>sequences</i> in the recombination site that can be used in gene-stacking or other multigenic cloning strategies. |