| 摘要 |
<p>The present invention is related to a method for the preparation of DNA (deoxyribonucleic acid) from biological materials. The method for preparation of DNA from biological materials describes pre-treatment of the raw materials prior to conventional methods for DNA preparation. The pre-treatment is mixing the raw material with either acid, organic solvent or a mixture of acid and organic solvent. The pre-treatment may be followed by filtration to remove soluble materials, lipid extraction using conventional organic solvents (e.g. ethanol) to remove lipids and when using acid the raw material should be neutralised before heating and boiling the mixture in an alkaline solution. By this pre-treatment other substances may be extracted from the raw material prior to DNA preparation, the filtering properties of the raw material mixture after boiling in alkaline are improved and the resulting yield of DNA is higher than without the pre-treatment of the raw material.</p> |
