NOVEL YEAST VARIANTS AND PROCESS FOR PRODUCING GLYCOPROTEIN CONTAINING MAMMALIAN TYPE SUGAR CHAIN

摘要

Novel yeast variants capable of producing a glycoprotein wherein a sugar chain having the same sugar chain structure as a sugar chain produced by mammalian cells is attached to an asparagine residue of a protein; and a process for producing the sugar chain and the glycoprotein by a sugar chain engineering technique with the use of these variants. By using the auxotrophic triplex or tetraplex variant newly bred, a neutral sugar chain identical with the high-mannose type produced by mammalian cells (human cells, etc.) or a glycoprotein having the same neutral sugar chain can be efficiently produced at a high purity. By transferring a mammalian type sugar chain biosynthesis-associated gene into such a variant, it is also possible to efficiently produce a mammalian sugar chain of high-mannose type, hybrid type, complex type, etc. or a protein having a mammalian type sugar chain.