CENTRIFUGAL MICROFLUIDIC PLATFORM FOR AUTOMATED MEDIA EXCHANGE

摘要

A microfluidic device and method comprises a rotatable substrate configured to be rotated at various angular velocities in order to accomplish a series of media exchanges with a sample chamber for containing a sample. A plurality of media reservoirs and waste reservoirs are connected to the sample chamber via channels forming capillary valves in which the capillary valves have varying burst frequencies of rotation of the substrate. The substrate can then be rotated through a series of angular velocities to automate media exchanges between the sample chamber and the respective media reservoirs and waste reservoirs. A pair of media reservoirs are located radially inward of the sample chamber and a pair of waste reservoirs are located radially outward of the sample chamber in an “X” configuration such that centrifugal forces cause fluid media to open the capillary valves and flow among the sample chamber, media reservoirs and waste reservoirs.

主权项

1. A microfluidic device, comprising:
a substrate configured for rotation about an axis of rotation, the substrate having formed therein a media exchange module, the media exchange module comprising:
a sample chamber;a first waste reservoir located radially outward of the sample chamber and connected to the sample chamber via a first channel, the first channel forming a first capillary valve having a first burst frequency;a first media reservoir located radially inward of the sample chamber and connected to the sample chamber via a second channel, the second channel forming a second capillary valve having a second burst frequency which is greater than the first burst frequency;a second waste reservoir located radially outward of the sample chamber and connected to the sample chamber via a third channel, the third channel forming a third capillary valve having a third burst frequency which is greater than the second burst frequency; anda second media reservoir located radially inward of the sample chamber and connected to the sample chamber via a fourth channel, the fourth channel forming a fourth capillary valve having a fourth burst frequency which is greater than the third burst frequency.