发明名称 |
METHODS AND MATERIALS FOR THE GROWTH OF PRIMATE-DERIVED PRIMORDIAL STEM CELLS |
摘要 |
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt. |
申请公布号 |
EP1025204(A4) |
申请公布日期 |
2001.02.28 |
申请号 |
EP19980956192 |
申请日期 |
1998.10.23 |
申请人 |
GERON CORPORATION |
发明人 |
BODNAR, ANDREA, G.;CHIU, CHOY-PIK;GOLD, JOSEPH, D.;INOKUMA, MARGARET;MURAI, JAMES, T.;WEST, MICHAEL, D. |
分类号 |
C12N15/09;C12N5/02;C12N5/0735;(IPC1-7):C12N5/00;C12N5/06;C12N5/10;C12N15/00;C12N15/01 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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