| 摘要 |
In a process and a device for the parallel preparation of at least 4n oligonucleotides, at least four inserts each with n reaction vessels are first arranged on a plate (16), each reaction vessel containing a nucleotide initiator base bound to an inert carrier. Particular operations are then carried out in parallel with one another at four stations (28, 30, 32, 34), and in particular a deblocking operation simultaneously in all n reaction vessels of the insert at the first station (28), a first washing operation simultaneously in all n reaction vessels of the insert at the second station (30), a coupling operation in all n reaction vessels of the insert at the third station (32), and, simultaneously in all n reaction vessels of the insert at the fourth station (34), a second washing operation followed by a capping operation followed by a third washing operation followed by an oxidation operation followed by a fourth washing operation. The plate (16) with the inserts is rotated station by station, the above-mentioned operations being carried out, until the desired oligonucleotides have been formed by coupling individual nucleotides to one another. |
