| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of analyzing a gene by improving the analysis rate by means of immobilizing either a probe nucleic acid or a sample nucleic acid containing a target sequence complementary to the sequence of this probe nucleic acid on a base plate and reacting these nucleic acids in the presence of a double strand DNA-binding protein. SOLUTION: On the gene analysis containing the detection of hybridization between a probe nucleic acid and a sample nucleic acid containing a target sequence complementary to the sequence of this probe nucleic acid, either the above probe nucleic acid or the sample nucleic acid is immobilized on a base plate and at least one of the above these nucleic acids is DNA. By forming the above hybridization in the presence of a double strand DNA-binding protein, e.g. Sulfolobus solfataricus-derived Sso7d protein or the like, the gene is analyzed rapidly with high sensitivity. This method can be utilized in the determination of the base sequence of nucleic acid, the gene diagnosis of infectious disease or hereditary disease, the expression monitoring of genomic gene, or the like. |
