| 摘要 |
PROBLEM TO BE SOLVED: To count microorganisms having a characteristic shape such as a circular shape, a curved shape and a linear shape which are to become a colony. SOLUTION: The microscopic image of a microorganism is fetched, a hue being close to a circular microorganism is extracted from an input image, by using a hue model for the circular microorganism and is made variable density, so as to easily detect a change point (S11 to S13). The changed point (edge) of variable density is extracted, by performing second derivative of the variable density for every pixel (S14), a circle or a circular arc composed of three points being endpoints and a midpoint of an edge point detected as a point sequence, and the color components of an area in the circular arc are taken by each pixel (S15). The upper and lower limit allowable values of the average value of hue of an erroneous area are calculated, the area (area of characteristic microorganism) A of the hues of an input image entered within the allowable range of the average value of the hue is calculated (S16), the area A and the single body area (a) of the microorganism are acquired experimentally, and the number of microorganisms is calculated, by using a coefficient (k) compensating the thickness of the microorganisms (S17). |
