IMPROVED KERATINOCYTE CULTURE AND USES THEREOF

摘要

The present invention relates to the treatment of skin defects by organotypically-cultured autologous keratinocytes isolated from the outer ro ot sheath of anagen or growing hair. Methods for primary, as well as subsequent organotypic cultures (i.e., epidermal equivalents) in fully-defined media supplemented by autologous human serum and substances isolated form blood components, with minimal allogeneic biological supplements, are disclosed herein. Techniques to prepare epidermal equivalents for transplantation by u se of a biocompatible glue are also disclosed herein.