发明名称 |
Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction |
摘要 |
The presence of a target in a test mixture or the concentration of such target can be determined by a method relying on fluorescence emission measurement. Such method utilizes a nucleic acid ligand to the target and a ligand beacon comprising: i) a nucleic acid sequence complimentary to at least a portion of said nucleic acid ligand, ii) a fluorescent group, and iii) a fluorescence-modifying group. The emission profile of the ligand beacon is altered based on the presence and/or concentration change of the target in the test mixture.
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申请公布号 |
US6177555(B1) |
申请公布日期 |
2001.01.23 |
申请号 |
US19990447863 |
申请日期 |
1999.11.23 |
申请人 |
NEXSTAR PHARMACEUTICALS, INC. |
发明人 |
JAYASENA SUMEDHA;GOLD LARRY |
分类号 |
C07H21/00;C12Q1/68;(IPC1-7):C07H21/04 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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