发明名称 |
METHOD FOR CONSTRUCTING FULL-LENGTH CDNA LIBRARIES |
摘要 |
A method whereby a cDNA library having an extremely high full-length ratio c an be constructed starting with a material in a very small amount compared with the conventional methods. This method comprises optimizing reaction conditio ns in the steps of the alkaline phosphatase reaction, acid pyrophosphatase reaction and RNA ligase reaction in the conventional oligocap method.</SDOAB >
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申请公布号 |
CA2387829(A1) |
申请公布日期 |
2001.01.18 |
申请号 |
CA20002387829 |
申请日期 |
2000.07.06 |
申请人 |
HELIX RESEARCH INSTITUTE |
发明人 |
OTSUKA, KAORU;ISOGAI, TAKAO;OTA, TOSHIO;SUGANO, SUMIO;SUZUKI, YUTAKA;WAKAMATSU, AI |
分类号 |
C12N15/10;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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