发明名称 |
Pyruvate decarboxylase for obtaining acyloins, process for preparing such pyruvate decarboxylase, DNA sequences of correspondingly encoding PDC-gene and process for obtaining acyloins |
摘要 |
The present invention relates to pyruvate decarboxylase (PDC) for transformation of pyruvate in the presence of benzaldehyde to (R)-(-)-phenylacetylcarbinol (product I) of >= 95 % enantiomeric purity with ratio of the product I to 2-hydroxypropiophenone >= 95 % and with specific activity for formation the product I grater than 1U/mg, whose tryptophan radical in position 392 is substituted with a radical of a natural aliphatic amino acid, and further to a process for obtaining pyruvate decarboxylase (PDC) with specific activity greater than 1U/mg for formation of phenylacetylcarbinol, by isolating thereof from a production organism with a gene encoding PDC from Zymomonas mobilis, in the DNA sequence of which the TGG codon in position 1174-1176 encoding for tryptophan radical in position 392 is substituted with a codon encoding a radical of a natural aliphatic amino acid. In the present invention there is also claimed a process of enzymatic preparation of acyloins by enzymatic acyloin condensation of {alpha}-ketocarbonic acids and/or aldehydes in the presence of pyruvate decarboxylase (PDC) whose tryptophan radical in position 392 is substituted with a radical of a natural aliphatic amino acid. |
申请公布号 |
CZ287619(B6) |
申请公布日期 |
2001.01.17 |
申请号 |
CZ19970003691 |
申请日期 |
1996.05.22 |
申请人 |
FORSCHUNGSZENTRUM JUELICH GMBH;BRUHN HEIKE;POHL MARTINA;MESCH KATRIN;KULA MARIA REGINA |
发明人 |
BRUHN HEIKE;POHL MARTINA;MESCH KATRIN;KULA MARIA REGINA |
分类号 |
C12N15/09;C12N9/02;C12N9/88;C12N15/00;C12N15/60;C12P7/26;C12P7/42;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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